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Analysis of PSD-95 (Human, Mouse, Rat) by ELISA (CAT#: STEM-MB-1143-LGZ)

Introduction

Postsynaptic density (PSD) is a special structure located in the postsynaptic membrane of the central nervous system, which is a key substance in postsynaptic signal transduction and integration. PSD-95 has no protease activity. It mainly combines with related receptors and signal molecules through different structural domains to form signal complexes, which have various biological functions, such as participating in the formation of synaptic connections, maintaining synaptic plasticity and participating in pain regulation.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling, Phosphoproteins

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and tissue
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