Analysis of PSMD4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0610-LGZ)

Introduction

Official Full Name: proteasome 26S subunit ubiquitin receptor, non-ATPase 4
Also known as: AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
The 26S proteasome is a multicatalytic protease complex with a highly ordered structure consisting of 2 complexes, a 20S core and a 19S regulator. The 20S core consists of 4 rings composed of 28 different subunits; 2 rings composed of 7 α subunits, and 2 rings composed of 7 β subunits. The 19S regulator consists of a base (containing 6 ATPase subunits and 2 non-ATPase subunits) and a lid (containing up to 10 non-ATPase subunits). The proteasome is distributed in high concentrations in eukaryotic cells and cleaves peptides in a non-lysosomal pathway in an ATP/ubiquitin-dependent process. A modified proteasome, the essential function of the immunoproteasome is to process MHC class I peptides. This gene encodes a non-ATPase subunit of the 19S regulatory lid. Pseudogenes were identified on chromosomes 10 and 21.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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