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Analysis of PSP-D (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0511-LGZ)

Introduction

PSP-D pulmonary surfactant protein, whose gene is located on the long arm of chromosome 10, is a macromolecular hydrophilic glycoprotein and belongs to the large family of calcium-dependent C-type lectins. PSP-D is composed of multimers, and there are different multimer forms among different species and different individuals. It plays an important role in maintaining the normal structure and function of the pulmonary surfactant phospholipids in the alveolar cavity, and in regulating local immunity and inflammation.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer, Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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