Analysis of PTH Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2444-LGZ)

Introduction

Official Full Name: parathyroid hormone<br />Also known as: FIH1; PTH1<br />This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of C1orf53 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0262-LGZ)
Analysis of Macrobrachium Rosenbergii Nodavirus (MrNV) by Real-Time PCR Method (CAT#: STEM-MB-3154-LGZ)
Analysis of Cytoplasmic Polyhedrosis Virus (CPV) by Real-Time PCR Method (CAT#: STEM-MB-2592-LGZ)
Analysis of HNRNPU Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1043-LGZ)
Analysis of SNHG7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1984-LGZ)
Analysis of PHPT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1971-LGZ)
Analysis of EIF4E2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1763-LGZ)
Analysis of PKLR Gene (Mutation) in Pyruvate Kinase Deficiency of Red Cells by RT-qPCR (CAT#: STEM-MT-0145-LGZ)