Analysis of PTPN14 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0608-LGZ)

Introduction

Official Full Name: protein tyrosine phosphatase non-receptor type 14<br />Also known as: PEZ; PTP36; PTPD2; CATLPH<br />The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTP is known to be a signaling molecule that regulates a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal non-catalytic domain similar to that of proteins associated with the 4.1 superfamily cytoskeleton, suggesting that the protein localizes to the membrane or to the cytoskeleton. It appears to regulate lymphatic development in mammals, and a loss of function mutation has been found in a kindred with a lymphedema-choanal atresia.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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