Analysis of PTPRN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1518-LGZ)

Introduction

Official Full Name: protein tyrosine phosphatase receptor type N<br />Also known as: IA2; IA-2; ICA512; R-PTP-N; IA-2/PTP<br />The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTP is known to be a signaling molecule that regulates a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has an extracellular domain, a single transmembrane domain and a single catalytic domain, thus representing a receptor-type PTP. This PTP was found to be an autoantigen reactive with sera from patients with insulin-dependent diabetes mellitus (IDDM) and thus may be a potential target of diabetic autoimmunity. Alternate splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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