Analysis of RAB4A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0347-LGZ)

Introduction

Official Full Name: RAB4A, member RAS oncogene family<br />Also known as: RAB4; HRES1; HRES-1; HRES-1/RAB4<br />This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Brachyspira Spp. by Real-Time PCR Method (CAT#: STEM-MB-2741-LGZ)
Analysis of SPATA21 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1235-LGZ)
Analysis of NMNAT2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0482-LGZ)
Analysis of MIR199A2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0450-LGZ)
Analysis of Porcine Rotavirus Group B by Real-Time PCR Method (CAT#: STEM-MB-3327-LGZ)
Analysis of HJV Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0135-LGZ)
Analysis of B3GALT6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1262-LGZ)
Analysis of CDH23 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2075-LGZ)