Analysis of RAD50 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1846-LGZ)

Introduction

Official Full Name: RAD50 double strand break repair protein
Also known as: NBSLD; RAD502; hRad50
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in the repair of DNA double-strand breaks. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays the enzymatic activity required for the joining of many non-homologous DNA ends. The protein, in cooperation with its partners, plays an important role in DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Mouse homologous gene knockout studies have shown that this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakdown syndrome-like disorder.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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