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Analysis of RAGE (soluble) (Human) by ELISA (CAT#: STEM-MB-1152-LGZ)

Introduction

RAGE is a membrane protein that belongs to the immunoglobulin superfamily. It is highly homologous to MUC18 glycoprotein of the immunoglobulin superfamily, nerve cell adhesion molecule (NCAM), and the amino acid sequence of the intracellular part of CD20. Soluble RAGE(SRAGE), as the extracellular segment of RAGE, is a ligand-binding site with a V-type fragment followed by two C-type soluble immunoglobulin-like structures, each containing a pair of conserved cysteic acid residues, and soluble V-type soluble RAGE(SRage) contains two glycosylation sites coupled with N. These are important for the structural stability of RAGE and the function of specific recognition ligands.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Plasma, Serum

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