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Analysis of RAGE/STK30/MOK by ELISA (CAT#: STEM-MB-1595-LGZ)

Introduction

Advanced glycation end product receptor (RAGE) is a 35kDa immunoglobular superfamily transmembrane receptor that derives its name from its ability to bind to advanced glycation end products. RAGE is generally considered a class of recognition receptors because of its inflammatory function in innate immunity and its ability to recognize a class of ligand by a common motif. Compared with other tissues, RAGE was most expressed in the lungs, especially in type I alveolar epithelial cells, but not in idiopathic pulmonary fibrosis (IPF), suggesting that the expression and regulation of RAGE in the lung system is different from that in the cardiovascular system. Blocking/knocking out RAGE resulted in impaired cell adhesion and increased cell proliferation and migration.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

RAGE is associated with some chronic diseases, such as atherosclerosis, peripheral vascular disease, myocardial infarction, congestive heart failure, diabetes, Alzheimer's disease, etc.

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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