Analysis of RANTES (Human) by ELISA (CAT#: STEM-MB-1155-LGZ)

Introduction

RANTES/CCL5 (regulated upon activation, expressed and secreted in T cells) is a member of the "C-C" or beta chemokine family that induces inflammation and has been implicated in a variety of inflammatory diseases. RANTES is primarily produced and secreted by CD8+ T cells, macrophages, platelets, epithelial cells, fibroblasts, and certain tumor cells. RANTES is a chemoattractant with additional regulatory functions on a variety of cell types including monocytes, memory T cells, natural killer cells, eosinophils, basophils, dendritic cells and mast cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum

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