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Analysis of RBM8A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0364-LGZ)

Introduction

Official Full Name: RNA binding motif protein 8A
Also known as: TAR; Y14; RBM8; ZNRP; RBM8B; ZRNP1; BOV-1A; BOV-1B; BOV-1C; MDS014; DEL1q21.1; C1DELq21.1
This gene encodes a protein with a conserved RNA-binding motif. This protein is mainly found in the nucleus, but is also present in the cytoplasm. It is preferentially associated with mRNAs generated by splicing, including nuclear mRNAs and newly exported cytoplasmic mRNAs. The protein is thought to remain associated with the spliced mRNA as a marker to indicate where introns are present, thereby coupling pre- and post-splicing events in the mRNA. Previously, it was believed that there were two genes encoding this protein: RBM8A and RBM8B; the RBM8B locus is now considered to be a pseudogene. The gene has two alternating translation initiation codons, resulting in two forms of the protein. Allelic mutations and low frequency noncoding single nucleotide polymorphisms (SNPs) of this gene cause thrombocytopenia-deleted radius (TAR) syndrome.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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