Analysis of RD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0332-LGZ)

Introduction

Official Full Name: RD3 regulator of GUCY2D<br />Also known as: LCA12; C1orf36<br />This gene encodes a retinal protein that is associated with promyelocytic leukemia-gene product (PML) bodies in the nucleus. Mutations in this gene cause Leber congenital amaurosis type 12, a disease that results in retinal degeneration. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of TMEM63A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0728-LGZ)
Analysis of Salmonella Choleraesuis by Real-Time PCR Method (CAT#: STEM-MB-3399-LGZ)
Analysis of Washington University Polyomavirus (WUPyV) by Real-Time PCR Method (CAT#: STEM-MB-3903-LGZ)
Analysis of Bartonella Quintana by Real-Time PCR Method (CAT#: STEM-MB-3367-LGZ)
Analysis of Perkinsus Marinus by Real-Time PCR Method (CAT#: STEM-MB-3629-LGZ)
Analysis of Canine Parvo Virus (CPV) by Real-Time PCR Method (CAT#: STEM-MB-3034-LGZ)
Analysis of Epstein Barr Virus (EBV) by Real-Time PCR Method (CAT#: STEM-MB-2992-LGZ)
Analysis of RCOR3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0337-LGZ)