Analysis of Reg-3-α (Human) by ELISA (CAT#: STEM-MB-1157-LGZ)

Introduction

Reg genes mainly in gastrointestinal tissues and injury in the pancreas increased regeneration is the ability to promote islet β cell tissue regeneration genes, in recent years, research on Reg mainly concentrated in the Reg various subtypes of genetic structure, and its application in diabetes, gastrointestinal cancer, inflammation, and play a role in antibacterial and related mechanisms. RegⅢ(Reg3) plays a more prominent role in various subtypes of Reg, which is expected to be an effective target for the treatment of these diseases

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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