Analysis of Renin (Human) by ELISA (CAT#: STEM-MB-1159-LGZ)

Introduction

Renin, also known as angiotensinogen enzyme, is involved in the renin-angiotensin system (RAS) of the body. The expression gene of renin is located in 1q32.1, and the main function of Renin is to cause increased blood pressure and intrarenal recovery of perfusion pressure. Renin catalyzes the first step in the angiotensinogen activation pathway, a series of reactions that eventually lead to aldosterone release and vasoconstriction. Renin cleaved angiotensinogen to produce angiotensin I through rate-limiting reaction, and then was catalyzed by angiotensin I converting enzyme to produce angiotensin II. RAS plays an important role in regulating blood pressure and maintaining electrolyte balance.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiac Biomarkers, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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