Analysis of Resistin (Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0525-LGZ)

Introduction

Resistin, also known as FIZZ3 or ADSF, belongs to the family of resistin-like molecules (RELMs), and is related to obesity and insulin resistance. The amount of newly synthesized MMPs is mainly controlled by the level of replication. The activity of MMPs is mainly regulated by the activation of zymogens and their inhibitory proteins (TIMPs). RESISTIN is a true collagenase that can degrade type Ⅰ, Ⅱ, and Ⅲ collagenase.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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