Analysis of Rhodococcus Equi by Real-Time PCR Method (CAT#: STEM-MB-3815-LGZ)

Introduction

Rhodococcus equi (formerly Corynebacterium equi) is a pleomorphic gram-positive rod that is a normal inhabitant of soil and can be cultured from horse feces. It causes a pyogranulomatous pneumonia in foals aged 2 to 6 months that are living on endemic farms.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.