Analysis of RHOU Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0617-LGZ)

Introduction

Official Full Name: ras homolog family member U
Also known as: ARHU; G28K; WRCH1; hG28K; CDC42L1
This gene encodes a member of the Rho family of gtpases. This protein activates PAK1 and JNK1 and induces filopodia formation and stress fibril lysis. It may also mediate the role of WNT1 signaling in regulating cell morphology, cytoskeletal organization and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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