Analysis of RORC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0642-LGZ)

Introduction

Official Full Name: RAR related orphan receptor C
Also known as: TOR; RORG; RZRG; IMD42; NR1F3; RZR-GAMMA
The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Actinomadura Pelletieri by Real-Time PCR Method (CAT#: STEM-MB-3640-LGZ)
Analysis of Prolactinomas by RT-qPCR (CAT#: STEM-MT-0003-LGZ)
Analysis of Yellow Head Virus(YHV) by Real-Time PCR Method (CAT#: STEM-MB-3622-LGZ)
Analysis of OR52E2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2340-LGZ)
Analysis of CPT2 Gene (Mutation) in Carnitine Palmitoyltransferase II Deficiency by RT-qPCR (CAT#: STEM-MT-0109-LGZ)
Analysis of MED30 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1902-LGZ)
Analysis of SAP130 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1699-LGZ)
Analysis of RXRG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0341-LGZ)