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Analysis of RPL22 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1409-LGZ)

Introduction

Official Full Name: ribosomal protein L22
Also known as: EAP; L22; HBP15; HBP15/L22
The ribosome is an organelle that catalyzes protein synthesis and consists of a small 40S subunit and a large 60S subunit. These subunits consist of 4 RNAs and about 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. This protein belongs to the L22E family of ribosomal proteins. Its initial methionine residue is removed post-translationally. This protein can specifically bind Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. This mouse protein has been shown to bind heparin. Transcript variants exist utilizing alternative polyA signals. As a typical gene encoding ribosomal proteins, multiple processing pseudogenes of this gene are scattered throughout the genome. This gene was previously thought to be located at 3q26 and is fused to the acute myeloid leukemia 1 (AML1) gene at 21q22 in some patients with treatment-related myelodysplastic syndrome. However, these fusions actually involve the ribosomal protein L22 pseudogene located at 3q26, which actually maps to 1p36.3-p36.2.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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