Analysis of RPL27A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2129-LGZ)

Introduction

Official Full Name: ribosomal protein L27a
Also known as: L27A; uL15
The ribosome is an organelle that catalyzes protein synthesis and consists of a small 40S subunit and a large 60S subunit. These subunits consist of 4 RNAs and about 80 structurally distinct proteins. The ribosomal protein encoded by this gene is a component of the 60S subunit. This protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Altered expression of this gene has been observed in colorectal cancer compared with adjacent normal tissue, although no correlation between expression levels and disease severity was found. As a typical gene encoding ribosomal proteins, multiple processing pseudogenes from this gene are scattered throughout the genome.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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