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Analysis of RPLP2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2128-LGZ)

Introduction

Official Full Name: ribosomal protein lateral stalk subunit P2
Also known as: P2; LP2; RPP2; D11S2243E
The ribosome is an organelle that catalyzes protein synthesis and consists of a small 40S subunit and a large 60S subunit. These subunits consist of 4 RNAs and about 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. This protein is the functional equivalent of the Escherichia coli L7/L12 ribosomal protein and belongs to the L12P ribosomal protein family. It plays an important role in the elongation step of protein synthesis. Unlike most basic ribosomal proteins, this encoded protein is acidic. Its C-terminus is almost identical to that of the ribosomal phosphorylated proteins P0 and P1. P2 proteins can interact with P0 and P1 to form a pentameric complex consisting of P1 and P2 dimers and P0 monomers. This protein is located in the cytoplasm. As a typical gene encoding ribosomal proteins, multiple processing pseudogenes of this gene are scattered throughout the genome.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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