Analysis of RPS20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1869-LGZ)

Introduction

Official Full Name: ribosomal protein S20
Also known as: S20; uS10
The ribosome is an organelle that catalyzes protein synthesis and consists of a small 40S subunit and a large 60S subunit. These subunits consist of 4 RNAs and about 80 structurally distinct proteins. The ribosomal protein encoded by this gene is a component of the 40S subunit. This protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54 located on its second intron. As a typical gene encoding ribosomal proteins, multiple processing pseudogenes of this gene are scattered throughout the genome. Two transcript variants encoding different isoforms of this gene have been identified.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Fructus Piperis Longi by Real-Time PCR Method (CAT#: STEM-MB-3940-LGZ)
Analysis of TSPAN31 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2500-LGZ)
Analysis of NIBAN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0487-LGZ)
Analysis of ZBTB17 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1430-LGZ)
Analysis of Mycobacterium Africanum by Real-Time PCR Method (CAT#: STEM-MB-3711-LGZ)
Analysis of CNTFR Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2048-LGZ)
Analysis of INAVA Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1064-LGZ)
Analysis of YOD1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0292-LGZ)