Analysis of S100A5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0331-LGZ)

Introduction

Official Full Name: S100 calcium binding protein A5
Also known as: S100D
The protein encoded by this gene is a member of the S100 protein family, which contains two EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a variety of cells and are involved in the regulation of many cellular processes, such as cell cycle progression and differentiation. The S100 gene includes at least 13 members, which are located on chromosome 1q21. The Ca2+ affinity of this protein is 20-100 times higher than other S100 proteins studied under the same conditions. The protein also binds Zn2+ and Cu2+, Cu2+ strongly weakens the binding of Ca2+. This protein is expressed in a very limited area of the adult brain.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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