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Analysis of S100A8/MRP8 (Human) by ELISA (CAT#: STEM-MB-1163-LGZ)

Introduction

S100 calcium-binding protein A8 (S100A8), also known as calgranulin A (CAGA), is a protein encoded by the S100A8 gene and is a member of the S100 family of proteins containing two EF-hand calcium-binding motifs. It and S100A9 form a heterodimer called calprotectin. It is mainly derived from immune cells such as neutrophils and macrophages. There is some evidence that it can inhibit the precipitation of calcium oxalate in the environment of urine samples at its subphysiological concentrations. It may also function in the body as an inhibitor of calcium oxalate kidney stone formation. It also has antimicrobial potential due to its ability to bind zinc. It plays an important role in protecting the body from pathogenic infections, and its overexpression amplifies the inflammatory response, accelerating the release of more cytokines from neutrophils and macrophages, thereby inducing a vicious cycle and exacerbating the disease.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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