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Analysis of S100B (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0528-LGZ)

Introduction

S100B protein (S100B), also called S100 calcium-binding protein B, is a protein of the S-100 protein family, located on chromosome 21q22.3. It is glial-specific and is expressed primarily by but not all astrocytes, it is only expressed by the subtype of mature astrocytes that wrap the sheath vessels and by cells expressing NG2. This protein may play a role in neurite extension, melanoma cell proliferation, stimulation of Ca2+ flux, PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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