Analysis of Sagiyama Virus by Real-Time PCR Method (CAT#: STEM-MB-3620-LGZ)

Introduction

Sagiyama virus (Getah virus) is a member of the Semliki Forest virus antigenic complex of Old World alphaviruses. Sagiyama virus, which is closely related to Ross River virus, is relatively widely distributed, extending from Russia to South and Far Eastern Asia, the Pacific islands, and Australia.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.