Analysis of Salbutamol by ELISA (CAT#: STEM-MB-2248-LGZ)

Introduction

Salbutamol, a short-acting beta-2 adrenergic receptor agonist, used as an asthmatic. Adding a trace amount of salbutamol into livestock feed can increase lean meat volume and meat exchange rate of livestock and reduce fat, but its toxicity is much higher than ractopamine which has the same function. It was therefore forbidden to be used in large quantities. The new generation of drug residue detection products developed by ELISA technology has the characteristics of rapid, simple, accurate and high sensitivity compared with the instrumental analysis technology, and can minimize the operating error and working intensity.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

For food safety.

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Muscle and liver tissue, urine sample, serum, feed

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