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Analysis of SCF (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0536-LGZ)

Introduction

Stem cell factor (SCF), also known as c-kit ligand, is a basic hematopoietic stem cell growth factor with anti-apoptotic function. The human SCF gene encodes a leader peptide, an extracellular portion of 189 amino acids, a hydrophobic transmembrane region and an intracellular region. Natural SCF exists in a membrane-bound form or in a soluble form. The soluble portion is 164 or 165 amino acids from the beginning of the extracellular portion.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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