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Analysis of SCFR/Kit (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0537-LGZ)

Introduction

The proto-oncogene c-KIT is the gene encoding a receptor tyrosine kinase protein known as protein tyrosine kinase KIT, CD117 (cluster of differentiation 117), or mast/stem cell growth factor receptor (SCFR). Multiple transcript variants of this gene have been found, encoding different isoforms. KIT is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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