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Analysis of Schmallenberg Virus (SBV) by Real-Time PCR Method (CAT#: STEM-MB-2923-LGZ)

Introduction

A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.




Principle

Schmallenberg Virus (SBV) is an RNA virus with symptoms of fever, diarrhea, fatigue and so on. Dairy cows infected with this virus have reduced milk production and defects in newborn calves. At present, no evidence has been found that it can infect humans. Therefore, rapid and accurate identification of SBV is of great importance for prevention and quarantine of the disease. Primers and probes involved in this experiment have been optimized with high sensitivity. The primers are highly specific and are designed according to the highly conserved region of SBV, which will not cross-react with other viral Rnas.

Applications

For rapid and accurate identification of SBV.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: RNA