Analysis of Signal Transduction by Phospho Flow Cytometry (CAT#: STEM-CBT-0069-WXH)

Introduction

When a cell needs to convey a signal from receptors on the cell surface, or in response to conditions within the cell itself, post-translational modifications (PTMs) are often a key method of perpetuating that signal. The purpose of PTMs is generally to alter the activity, stability, or function of a protein, and principle among the PTMs is phosphorylation. Phosphorylation is the most well-studied modification used in cell signaling cascades.
Phospho-specific flow cytometry is an approach for analyzing and interpreting posttranslational modifications at the single-cell level. This innovation enables the simultaneous and high-throughput examination of two or more phospho-epitopes within multiple cell populations