Analysis of Simian T Lymphotropic Virus type 1 (STL V-1) by Real-Time PCR Method (CAT#: STEM-MB-3856-LGZ)

Introduction

Simian T-lymphotropic virus (STLV) is a C-type member of the oncornavirus subgroup of retroviruses. STLV was identified in the early 1980s, shortly after the initial isolation of HTLV-I, and represents the nonhuman primate counterpart of human T-lymphotropic viruses types I and II (HTLV-I, -II).
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.