Analysis of Sin Nombre Virus (SNV) by Real-Time PCR Method (CAT#: STEM-MB-3580-LGZ)

Introduction

Sin Nombre virus (SNV) was identified as the causative agent of Southwestern cases, and HPS has since been identified in 28 U.S. states, in Canada, and most recently in South America.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.