Analysis of SMCP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0324-LGZ)

Introduction

Official Full Name: sperm mitochondria associated cysteine rich protein
Also known as: MCS; MCSP; HSMCSGEN1
Sperm mitochondria differ from somatic cells in morphology and subcellular location. They are long and flat and form a helical coiled sheath in the middle of the sperm flagella. The protein encoded by this gene localizes to the sac associated with the outer mitochondrial membrane and is thought to play a role in the organization and stabilization of the mitochondrial sheath helix in sperm.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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