Analysis of SMYD2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0312-LGZ)

Introduction

Official Full Name: SET and MYND domain containing 2
Also known as: KMT3C; HSKM-B; ZMYND14
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Ornithostrongylus Quadriradiatus by Real-Time PCR Method (CAT#: STEM-MB-3582-LGZ)
Analysis of Anaplasma Phagocytophilum by Real-Time PCR Method (CAT#: STEM-MB-3488-LGZ)
Analysis of Salmonella Paratyphi C by Real-Time PCR Method (CAT#: STEM-MB-3409-LGZ)
Analysis of Measles Virus by Real-Time PCR Method (CAT#: STEM-MB-3160-LGZ)
Analysis of TRG-CCC1-1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1439-LGZ)
Analysis of CAPN2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0811-LGZ)
Analysis of Synechocystis sp. PCC6803 by RT-qPCR (CAT#: STEM-MT-0005-LGZ)
Analysis of Sindbis Virus (SINV) by Real-Time PCR Method (CAT#: STEM-MB-3581-LGZ)