Analysis of SOCS5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1750-LGZ)

Introduction

Official Full Name: suppressor of cytokine signaling 5<br />Also known as: CIS6; CISH6; Cish5; SOCS-5<br />The protein encoded by this gene contains an SH2 domain and a SOCS BOX domain. Thus, this protein belongs to the suppressor of cytokine signaling (SOCS) family, also known as the STAT-induced STAT inhibitor (SSI) protein family. Members of the SOCS family are known to be negative regulators of cytokine-induced cytokine signaling. The exact function of this protein has not been determined. Two alternatively spliced transcript variants encoding the same protein have been reported.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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