Analysis of Sodium (Na) by Enzyme-labeled Instrument (CAT#: STEM-MB-1457-LGZ)

Introduction

Sensitivity: 0.02 mmol/L
Detection range: 0.02 - 20 mmol/L
Precision: inter-lot difference of 8.4%, intra-lot difference of 2.2%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 405 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

The formation of nitrophenol from nitropyranoside as a substrate is catalyzed by β-galactosidase activated by sodium ions. The increase rate of absorption value of nitrophenol at 405 nm per unit time is directly proportional to sodium concentration.

Applications

Suitable for the determination of sodium ion concentration in serum (plasma).

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma

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