Analysis of SPINK-1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0550-LGZ)

Introduction

The protein encoded by the SPINK1 gene is a trypsin inhibitor secreted by pancreatic acinar cells into pancreatic juice. It is thought to play a role in preventing trypsin-catalyzed premature activation of zymogens in the pancreas and pancreatic ducts. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic, Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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