Analysis of SSR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0680-LGZ)

Introduction

Official Full Name: signal sequence receptor subunit 2
Also known as: TLAP; HSD25; TRAPB; TRAP-BETA
The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein (alpha-SSR or SSR1) and a 22-kD glycoprotein (beta-SSR or SSR2). The human beta-signal sequence receptor gene (SSR2) maps to chromosome bands 1q21-q23.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of REN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0599-LGZ)
Analysis of GPR153 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1332-LGZ)
Analysis of RIF1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1476-LGZ)
Analysis of Neisseria Gonorrhoeae by Real-Time PCR Method (CAT#: STEM-MB-3083-LGZ)
Analysis of Dhori Virus by Real-Time PCR Method (CAT#: STEM-MB-2584-LGZ)
Analysis of DISP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1294-LGZ)
Analysis of TMEM9B-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2147-LGZ)
Analysis of TRIM21 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2184-LGZ)