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Analysis of ST6GALNAC4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1951-LGZ)

Introduction

Official Full Name: ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 4
Also known as: IV; SIAT3C; SIAT7D; SIAT3-C; SIAT7-D; ST6GalNAc; ST6GALNACIV
The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from cmp-sialic acid to galactose-containing substrates. The encoded protein prefers glycoproteins rather than glycolipids as substrates and displays limited substrate specificity, utilizing only the trisaccharide sequence neu5ac-α-2,3-gal-β-1,3-galnac. In addition, it is also involved in the synthesis of ganglioside GD1A by GM1B. The encoded protein is normally found in the Golgi apparatus, but can be proteolytically processed to a soluble form. This protein is a member of the glycosyltransferase family 29. Transcript variants of this gene have been found to encode different isoforms. Readable transcripts exist for this gene and the downstream ST6GALNAC6 gene.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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