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Analysis of STIM1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2161-LGZ)

Introduction

Official Full Name: stromal interaction molecule 1<br />Also known as: GOK; TAM; TAM1; IMD10; STRMK; D11S4896E<br />This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by controlling store-operated Ca2+ influx channels (SOC). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenal adenocarcinoma, lung cancer, ovarian cancer, and breast cancer. This gene may play a role in malignancies and diseases involving this region, as well as in early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classical Kaposi's sarcoma, immunodeficiency due to fibroblast storage calcium entry deficiency (SOCE), ectodermal dysplasia, and tubular aggregate myopathy. The gene and ribonucleotide reductase 1 gene (RRM1) are in an end-to-end structure, and the 3' end of the gene is located at 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements