Analysis of Sucrose by Fluorescence Microplate Reader (CAT#: STEM-MB-1511-LGZ)

Introduction

Sensitivity: 0.15 μmol/L
Detection range: 0.15-15 μmol/L
Precision: inter-lot difference of 6.5%, intra-lot difference of 2.3%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 590 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Under acidic conditions, sucrose can be hydrolyzed into glucose by sucrose enzyme, which is catalyzed by glucose oxidase to produce hydrogen peroxide. Under the action of horseradish peroxidase, hydrogen peroxide can react with fluorescent probe to produce strong red fluorescent substance. The fluorescence intensity is linear with sucrose content within a certain range.

Applications

It is suitable for the determination of sucrose content in various plant tissues.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: various plant tissues

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