Analysis of Superoxide dismutases (Cu, Zn) by Raman Spectroscopy (CAT#: STEM-ST-0013-WXH)

Introduction

Superoxide dismutases (Cu, Zn) are cystosolic enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. Thus, they are an important antioxidant defense in nearly all cells exposed to oxygen. SOD is composed of the protein (apoenzym) and a catalytic prosthetic group in the form of metal atom serving as the active center. The ligands of the copper and zinc ions are six histidine molecules and one aspartate side chains, where one histidine molecule is shared between the two metal ions.

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Principle Applications Procedure Materials Notes Related Products

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

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