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Analysis of Syndecan-1 (Human) by ELISA (CAT#: STEM-MB-1192-LGZ)

Introduction

Syndecan-1 is a member of the adhesion molecule integrin transmembrane binder proteoglycan family. Through the side chain of heparin sulfate on its molecular surface, Syndecan-1 can bind a series of ligands, such as cell adhesion molecules, matrix components, growth factors, enzymes and enzyme inhibitors, to regulate the interaction between cells and the microenvironment in a co-receptor manner. It is involved in the regulation of a series of physiological processes such as the differentiation and development of tissues and organs, angiogenesis and tissue regeneration.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Metabolic, Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or supernatant