Analysis of TAF5L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0300-LGZ)

Introduction

Official Full Name: TATA-box binding protein associated factor 5 like
Also known as: PAF65B
The product of this gene belongs to the WD-repeat TAF5 protein family. This gene encodes a protein that is part of the PCAF histone acetylase complex. The PCAF histone acetylase complex consists of more than 20 polypeptides, some of which are TAFs, and are required for myogenic transcription and differentiation. TAFs may be involved in basal transcription, acting as coactivators, functioning in promoter recognition or modifying general transcription factors to facilitate complex assembly and transcription initiation. The encoded protein is structurally similar to TAF5, one of the histone-like TAFs. Alternative splicing transcript variants encoding different isoforms of this gene have been identified.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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