Analysis of Tau (pT231) (Human, Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0563-LGZ)

Introduction

Tau protein is a major component of neurofibrillary tangles and a marker for a range of neurodegenerative diseases, including Alzheimer's disease (AD). There are 6 isoforms of tau protein, and these 6 isoforms are often present in the PHF of AD in a hyperphosphorylated state.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Alzheimer’s Disease, Phosphoproteins, Neurobiology/Neurodegeneration, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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