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Analysis of TCAP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2487-LGZ)

Introduction

Official Full Name: titin-cap<br />Also known as: TELE; CMD1N; CMH25; T-cap; LGMD2G; LGMDR7; telethonin<br />Sarcomere assembly is regulated by the muscle protein titin. Myosin is a giant elastin protein with kinase activity that reaches up to half the length of a sarcomere. It is the scaffold to which myofibrils and other muscle-associated proteins attach. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domain and is a substrate for titin kinase, an interaction thought to be critical for sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements