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Analysis of TECK/CCL25 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0567-LGZ)

Introduction

Thymus-expressed chemokine (TECK; CCL25) is a small cytokine belonging to the CC chemokine family, also known as TECK (thymus-expressed chemokine). It is thought to play a role in the development of T cells. It is chemotactic for thymocytes, macrophages and dendritic cells. CCL25 acts by binding to the chemokine receptor CCR9. It is produced as a protein precursor containing 151 amino acids. The gene for CCL25 (scya25) is located on chromosome 19.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation, Cytokines & Chemokines

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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