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Analysis of TGF-β3 (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0580-LGZ)

Introduction

Transforming growth factor-β (TGF-β) belongs to a group of newly discovered TGF-β superfamily that regulates cell growth and differentiation. This cytokine transforms the phenotype of normal fibroblasts by altering their adherent growth characteristics in the presence of epidermal growth factor (EGF) to gain the ability to grow in AGAR without the density-dependent inhibition of growth.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Goat Polyclonal
Recombinant Standards: Expressed in E. coli

Applications

Cytokines & Chemokines, Oncology & Cancer, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Cell Culture Supernatant
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